Device

Part:BBa_K329009:Design

Designed by: Raphael Pantier   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-12)


attC - double terminator (B0015) - attC - mRFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 277
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 277
    Illegal NotI site found at 1153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 277
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 277
    Illegal AgeI site found at 856
    Illegal AgeI site found at 968
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS-mRFP is situated between the SpeI and PstI restriction sites, the excision of this gene permit to restore a normal biobrick suffix. We used BioBrick Standard Assembly.


Source

BBa_K329008

References